Controls-For-Icc-If-Experiments

Controls for ICC/IF Experiments . Counterstaining & Mounting. Useful Links. Multicolor ICC/IF Protocol. Troubleshooting ICC/IF. Secondary Antibodies. Organelle Markers Guide. View all protocols. ICC Handbook. What is the significance of fixation?, Controls for ICC/IF Experiments . There are several variables in ICC/IF which can contribute to background staining and false-positive results. To identify potential sources of non-specific staining, the appropriate controls should be included in each experiment. Listed below are some of the controls which are essential for ICC/IF experiments.